CGD1
Cell Growth Determination Kit, MTT based
Synonyme(s) :
Mitochondrial activity assay
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12352207
Service technique
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pkg of 1 kit
Quality Level
technique(s)
UV/Vis spectroscopy: suitable, protein quantification: suitable
Amax
570 nm
application(s)
cell analysis
detection
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
General description
Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.
Application
Cell Growth Determination Kit, MTT based has been used:
- to measure the cell viability of preosteoblastic cells
- in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
- to determine the cell viability of primary cervical epithelial cells
Packaging
Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
target_organs
Central nervous system
Classe de stockage
3 - Flammable liquids
flash_point_f
53.6 °F - closed cup
flash_point_c
12 °C - closed cup
wgk
WGK 3
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Contenu apparenté
Datasheet
Carla M R Lacerda et al.
American journal of physiology. Heart and circulatory physiology, 302(10), H1983-H1990 (2012-02-22)
This study addressed the following questions: 1) Does cyclic tensile strain induce protein expression patterns consistent with myxomatous degeneration in mitral valves? 2) Does cyclic strain induce local serotonin synthesis in mitral valves? 3) Are cyclic strain-induced myxomatous protein expression
Ahran Pae et al.
The journal of advanced prosthodontics, 6(2), 96-102 (2014-05-21)
This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate
Mengxiong Wang et al.
Oncogene, 38(22), 4264-4282 (2019-02-06)
While HER2 and EGFR are overexpressed in breast cancers and multiple other types of tumors, the use of EGFR and/or HER2 inhibitors have failed to cure many cancer patients, largely because cancers acquire resistance to HER2/EGFR-specific drugs. Cancers that overexpress