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Merck

A1420

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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A propos de cet article

NACRES:
NA.75
UNSPSC Code:
41161501
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form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

General description

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.

Preparation Note

Formulated to contain 8.8 grams of powder per liter of medium.
Supplement with 1.9 g/L sodium bicarbonate.

Classe de stockage

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Frans Vinberg et al.
Vision research, 101, 108-117 (2014-06-25)
The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
Antoine Chaffiol et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order
Qiuju Jiang et al.
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society, 26(5), 969-976 (2018-05-13)
Significant progress has been made recently in treating neurological blindness using implantable visual prostheses. However, implantable medical devices are highly invasive and subject to many safety, efficacy, and cost issues. The discovery that ultrasound (US) may be useful as a
Kasey Rose et al.
Molecular neurodegeneration, 12(1), 28-28 (2017-04-13)
Light exposure triggers movement of certain signaling proteins within the cellular compartments of the highly polarized rod photoreceptor cell. This redistribution of proteins between the inner and outer segment compartments affects the performance and physiology of the rod cell. In
Shijun Weng et al.
Journal of biological rhythms, 24(5), 391-402 (2009-09-17)
Intrinsically photosensitive retinal ganglion cells (ipRGCs) project to the suprachiasmatic nucleus (SCN) and are essential for normal photic entrainment of global circadian rhythms in physiology and behavior. The effect of light on the central clock is dependent on circadian phase

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