A2765
Azocasein
protease substrate, chromogenic, powder
Synonyme(s) :
Sulfanilamide-azocasein
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Laissez-nous vous aiderNom du produit
Azocasein, protease substrate
biological source
bovine
form
powder
technique(s)
ligand binding assay: suitable
solubility
water: 5 mg/mL, clear, orange to very deep orange
ε (extinction coefficient)
≥25 at 440 nm in 0.1 M NaOH at 1%
storage temp.
2-8°C
Quality Level
General description
Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.
Application
Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
Other Notes
View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Isabel Morales-Belpaire et al.
Water research, 42(17), 4449-4456 (2008-09-03)
Laboratory data on the behaviour of the pathogenic form of the prion protein (PrP(Sc)) in environmental matrices such as sewage sludge is scarce. Direct experiments with this misfolded protein require strict safety measures, pathogen class-3 facilities and costly reagents. However
Yosuke Tashiro et al.
Journal of bacteriology, 191(24), 7509-7519 (2009-10-20)
The opportunistic human bacterial pathogen Pseudomonas aeruginosa produces membrane vesicles (MVs) in its surrounding environment. Several features of the P. aeruginosa MV production mechanism are still unknown. We previously observed that depletion of Opr86, which has a role in outer
Isolation and characterization of chitinolytic Streptomyces sp. MT7 and its antagonism towards wood-rotting fungi
Nagpure, A, et al.
Annals of Microbiology, 64(2), 531-541 (2014)
Azocasein assay for alkaline protease in complex fermentation broth
Iversen SL and Jorgensen MH
Biotechnol. Tech., 9(8), 573-576 (1995)
Recombinant proteins from plants: production and isolation of clinically useful compounds, 3 (1998)
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