A2765
Azocasein
protease substrate, chromogenic, powder
Synonyme(s) :
Sulfanilamide-azocasein
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Laissez-nous vous aiderNom du produit
Azocasein, protease substrate
biological source
bovine
form
powder
technique(s)
ligand binding assay: suitable
solubility
water: 5 mg/mL, clear, orange to very deep orange
ε (extinction coefficient)
≥25 at 440 nm in 0.1 M NaOH at 1%
storage temp.
2-8°C
Quality Level
Application
Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
General description
Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.
Other Notes
View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hongfei Liu et al.
Protein and peptide letters, 27(11), 1102-1113 (2020-03-21)
Protein drugs have disadvantages, such as short half-lives, unstable biological activities, and low utilization efficiency. In this paper, a porous ion-responsive targeted drug delivery system was designed, combining biodegradable carriers with ion exchange technology to overcome problems for protein drug
Recombinant proteins from plants: production and isolation of clinically useful compounds, 3 (1998)
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International, 2016 (2016)
Nicolas Oswaldo Gomez et al.
Molecular microbiology, 115(1), 84-98 (2020-09-09)
To overcome the metal restriction imposed by the host's nutritional immunity, pathogenic bacteria use high metal affinity molecules called metallophores. Metallophore-mediated metal uptake pathways necessitate complex cycles of synthesis, secretion, and recovery of the metallophore across the bacterial envelope. We
Isabel Morales-Belpaire et al.
Water research, 42(17), 4449-4456 (2008-09-03)
Laboratory data on the behaviour of the pathogenic form of the prion protein (PrP(Sc)) in environmental matrices such as sewage sludge is scarce. Direct experiments with this misfolded protein require strict safety measures, pathogen class-3 facilities and costly reagents. However
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