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A3356

Anti-Beta (β)-Amyloid antibody

rabbit polyclonal

Synonyme(s) :

Anti-A4 AMYLOID PROTEIN, Anti-ABETA, Anti-ABPP, Anti-AD1, Anti-AMYLOID BETA A4, Anti-AMYLOID-BETA PROTEIN (ABP), Anti-APPI

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), WB
Citations:
9
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Nom du produit

Anti-β−Amyloid [22-35] antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~4 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect ELISA: 0.2-0.4 μg/mL using β-amyloid peptide (22-35), western blot: 0.25-0.5 μg/mL using β-amyloid peptide (1-40)

UniProt accession no.

P05067

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APP(351)
mouse ... App(11820)
rat ... App(54226)

General description

Rabbit anti-β-amyloid (22-35) antibody specifically recognizes β-amyloid (22-35), β-amyloid (25-35), β-amyloid (1-40) and β-amyloid (1-42) and does not react with β-amyloid (32-35) and β-amyloid (35-25) by ELISA. The antibody detects β-amyloid (1-40) by immunoblotting (approx. 4kDa). Staining of the β-amyloid (1-40) band in immunoblotting is specifically inhibited by the immunizing peptide.
Cleavage of the amyloid precursor protein (APP) by γ-secretase produces the small fragment amyloid β (Aβ) peptide. Cleavage of APP occurs mostly at residue 40 but to a lesser extent residue 42. The amyloid β peptide contains a ~100 kDa soluble N-terminal fragment, and intracellular C-terminal fragments (CTFs) bearing the complete Aβ domain.
Proteolytic cleavage of the amyloid precursor protein (APP) produces the small fragment amyloid β peptide. Cleavage of APP occurs mostly at residue 40 but to a lesser extent residue 42. Aggregates of amyloid β peptide are found deposited in the brains of Alzheimer′s patients.

Immunogen

synthetic peptide corresponding to amino acids 22-35 of human β-amyloid (1-40) fragment, conjugated to KLH. This sequence corresponds to amino acids 693-706 of the human amyloid precursor protein APP, and is identical in mouse and rat APP.

Application

Rabbit anti-β-amyloid (22-35) antibody can be used for ELISA at a concentration of 0.2-0.4μg/mL using β-amyloid peptide (22-35). The product can also be used for western blot at 0.25-0.5μg/mL using β-amyloid peptide (1-40).
Anti-β-Amyloid (22-35) antibody produced in rabbit has been used in:
  • western blot
  • dot-blot assay
  • indirect enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Secreted Aβ lead to synaptic and neuritic compromise and glial activation. Aggregates of amyloid β peptide are found deposited in the brains of Alzheimer′s patients. Aβ(25-35) and Aβ(22-35) fragments are highly toxic segments of β-amyloid peptides that promote inflammatory processes in astrocytes and fibrillary aggregation of Aβ, thus representing a promising therapeutic target for Alzheimer′s.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide and 1% bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000543471
0000515764
0000515764
0000543471
0000439615

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Contenu apparenté

Data Sheet - A3356

Instructions

Xinyi Li et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(50), 19423-19433 (2013-12-18)
Tissue-specific overexpression of the human systemic amyloid precursor transthyretin (TTR) ameliorates Alzheimer's disease (AD) phenotypes in APP23 mice. TTR-β-amyloid (Aβ) complexes have been isolated from APP23 and some human AD brains. We now show that substoichiometric concentrations of TTR tetramers
Mechanisms of transthyretin inhibition of $\beta$-amyloid aggregation in vitro
Li X, et al.
The Journal of Neuroscience, 33(50), 19423-19433 (2013)
An Overview of APP Processing Enzymes and Products
Chow VW, et al.
Neuromolecular Medicine, 12, 1-1 (2010)
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