A4914
Conjugué anticorps anti-IgG de lapin (molécule entière)-peroxydase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Goat Anti-Rabbit IgG (whole molecule)–HRP
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A propos de cet article
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
129
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Laissez-nous vous aiderbiological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit polyclonal IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary polyclonal anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the immunological analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is specific for all rabbit IgGs and does not bind to human serum proteins in ELISA.
Immunogen
Purified rabbit IgG
Application
Une co-immunoprécipitation et une analyse par western blot de lysats de cellules C33A ont été réalisées en utilisant de l′IgG anti-lapin de chèvre conjuguée à de la HRP comme anticorps secondaire.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used in sandwich ELISA (diluted 1:3000 in PBS-0.05% Tween 20) and immunoelectrophoresis. The product can also be used for western blotting applications (at 1:2000 dilutions in mouse liver homogenates , or in mouse embryonic fibroblasts ).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Other Notes
Antibody adsorbed with human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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