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Merck

A7420

Amyloglucosidase from Aspergillus niger

greener alternative

lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

Synonyme(s) :

1,4-α-D-Glucane glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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A propos de cet article

Numéro CAS:
9032-08-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-877-2
MDL number:
MFCD00081350
Numéro CE :
3.2.1.3 (BRENDA, IUBMB)
Specific activity:
30-60 units/mg protein (biuret)

Principaux documents

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form

lyophilized powder

specific activity

30-60 units/mg protein (biuret)

composition

protein, ≥80%

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

impurities

≤0.02% glucose

greener alternative category

, Enabling

storage temp.

−20°C

Quality Level

200

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Catégories apparentées

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Physical form

Lyophilized powder containing less than 0.02% glucose

Other Notes

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000500852
0000500854
0000500854
0000500852
0000488613

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of Product A7420,  Amyloglucosidase from Aspergillus niger?

    Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).

  6. Does Product A7420,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?

    The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.

  7. How can a solution of Product A7420,  Amyloglucosidase from Aspergillus niger, be prepared?

    A 1 mg/mL solution may be prepared in water.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yi-Chen Lin et al.
PloS one, 12(5), e0177115-e0177115 (2017-05-05)
It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific
Youngmi Kim et al.
Bioresource technology, 99(12), 5165-5176 (2007-11-09)
DDGS and wet distillers' grains are the major co-products of the dry grind ethanol facilities. As they are mainly used as animal feed, a typical compositional analysis of the DDGS and wet distillers' grains mainly focuses on defining the feedstock's
Lizanne Janssens et al.
Aquatic toxicology (Amsterdam, Netherlands), 193, 210-216 (2017-11-04)
Pesticides are causing strong decreases in aquatic biodiversity at concentrations assumed safe by legislation. One reason for the failing risk assessment may be strong differences in the toxicity of the active ingredient of pesticides and their commercial formulations. Sublethal effects
Alisa K Manning et al.
Frontiers in genetics, 11, 615-615 (2020-08-06)
Causal transcripts at genomic loci associated with type 2 diabetes (T2D) are mostly unknown. The chr8p23.1 variant rs4841132, associated with an insulin-resistant diabetes risk phenotype, lies in the second exon of a long non-coding RNA (lncRNA) gene, LOC157273, located 175
M Ramadas et al.
World journal of microbiology & biotechnology, 12(3), 267-271 (1996-05-01)
Amyloglucosidase (AMG) was produced by Aspergillus niger in solid-state fermentation (SSF), submerged fermentation (SmF) and an aqueous, two-phase system of polyethyleneglycol (PEG) and salt. In SSF, a fed-batch mode of operation gave a yield of 64 U/ml compared with 44
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