Accéder au contenu
Merck

B8434

Anti-BrdU antibody, Mouse monoclonal

clone BU-33, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-BrdU antibody produced in mouse, Anti Brdu, Brdu Antibody, Brdu Antibody - Anti-BrdU antibody, Mouse monoclonal, Anti-Bromodeoxyuridine

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164550
Conjugate:
unconjugated
Clone:
BU-33, monoclonal
Application:
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Species reactivity:
wide range
Citations:
185
Technique(s):
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Anti-BrdU antibody, Mouse monoclonal, clone BU-33, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

BU-33, monoclonal

form

buffered aqueous solution

species reactivity

wide range

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Anti-BrdU antibody has been used in:
  • BrdU labeling
  • immunohistochemistry
  • immunostaining
  • BrdU assay
Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:
  • to detect 5-fluorouridine-labelled RNA
  • to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
  • against BrdU labelled rat retinal sections

Biochem/physiol Actions

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Immunogen

bromodeoxyuridine conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000488148
0000488149
0000488149
0000488148
0000462551

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Does 5-bromo-2'-deoxyuridine (BrdU) disrupt cell proliferation and neuronal maturation in the adult rat hippocampus in vivo?.
Hancock A, et al.
Behavioural Brain Research, 199(2), 218-221 (2009)
Regulation of later neurogenic stages of adult-derived neural stem/progenitor cells by L-type Ca2+ channels.
Teh DB, et al.
Development, Growth & Differentiation, 56(8), 583-594 (2014)
Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure.
Hong J, et al.
Experimental Eye Research, 111, 17-26 (2013)
Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure
Hong Jie, et al.
Experimental Eye Research, 17-26 (2013)
JAZ (Znf346), a SIRT1-interacting protein protects neurons by stimulating p21 (WAF/CIP1) expression.
Mallick S and D'Mello SR
The Journal of Biological Chemistry, jbc-M114 (2014)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique