C1117
Anti-Cy5 antibody, Mouse monoclonal
clone CY5-15, purified from hybridoma cell culture
Synonyme(s) :
Cy5 Antibody, Cy5 Antibody - Anti-Cy5 antibody, Mouse monoclonal, Monoclonal Anti-Cy5 antibody produced in mouse
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A propos de cet article
Conjugate:
unconjugated
Clone:
CY5-15, monoclonal
Application:
ARR, DB, ELISA (d), ICC, IP
Technique(s):
direct ELISA: suitable, dot blot: 1-2 μg/mL using cell protein extracts labeled with Cy5, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable
Citations:
6
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Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CY5-15, monoclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
concentration
~1.5 mg/mL
technique(s)
direct ELISA: suitable, dot blot: 1-2 μg/mL using cell protein extracts labeled with Cy5, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
Anti-Cy5 antibody, Mouse monoclonal has been used in immunofluorescence assays and western blotting.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody is used to tag CyDye 5 for detection and quantitation and signal applification by or within immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoprecipitation, immunocytochemistry, dot blot assay, and protein microarrays. Anti-Cy5 antibody is an important tool for Cy5 signal amplification in assays where biomolecules are labeled with Cy5.
Biochem/physiol Actions
Monoclonal Anti-Cy5 recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
The antibody recognizes Cy5 and AlexaFluor 647 labeled proteins, but not Cy3 labeled proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution and have tolerance to DMSO. They are more photo-stable than fluorescein, have high molar extinction coefficients, and favorable quantum yields. Due to their physical and chemical properties, they are used (mainly Cy3 and Cy5) in many different biological assays such as: DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
Immunogen
mixture of proteins labeled with Cy5.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Dissecting the role of COPI complexes in influenza virus infection
Sun E, et al.
Journal of Virology, 87(5), 2673-2685 (2013)
Talia D Valentini et al.
Nature communications, 11(1), 2287-2287 (2020-05-10)
Culture-independent studies of cystic fibrosis lung microbiota have provided few mechanistic insights into the polymicrobial basis of disease. Deciphering the specific contributions of individual taxa to CF pathogenesis requires comprehensive understanding of their ecophysiology at the site of infection. We
Eileen Sun et al.
Journal of virology, 87(5), 2673-2685 (2012-12-21)
As an obligate pathogen, influenza virus requires host cell factors and compartments to mediate productive infection and to produce infectious progeny virus. Recently, several small interfering RNA (siRNA) knockdown screens revealed influenza virus host dependency proteins, all of which identified
Jiang He et al.
PLoS pathogens, 9(10), e1003701-e1003701 (2013-10-17)
As an obligatory pathogen, influenza virus co-opts host cell machinery to harbor infection and to produce progeny viruses. In order to characterize the virus-host cell interactions, several genome-wide siRNA screens and proteomic analyses have been performed recently to identify host
Masaaki Kuwajima et al.
PloS one, 15(1), e0226797-e0226797 (2020-01-16)
Analysis of neuronal compartments has revealed many state-dependent changes in geometry but establishing synapse-specific mechanisms at the nanoscale has proven elusive. We co-expressed channelrhodopsin2-GFP and mAPEX2 in a subset of hippocampal CA3 neurons and used trains of light to induce
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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