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Merck

C3117

Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse

~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Monoclonal Anti-Cy3/Cy5

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A propos de cet article

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
CY-96, monoclonal
Application:
direct ELISA
dot blot
immunocytochemistry
microarray
Technique(s):
direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable
Citations:
13

Principaux documents

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Nom du produit

Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse, ~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CY-96, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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Application

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • dot blot assay
  • protein microarrays
  • immunofluorescenc

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

Biochem/physiol Actions

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
The antibody recognizes Cy3 and Cy5 conjugated to proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

Immunogen

mixture of proteins labeled with Cy3/Cy5.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000508172
0000508172
0000410527
0000410527
0000244190

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Consulter la Bibliothèque de documents

Wei Li et al.
Animal models and experimental medicine (2023-08-11)
The effect of platelet factor 4 (PF4) on bone marrow mesenchymal stem cells (BMMSCs) and osteoporosis is poorly understood. Therefore, this study aimed to evaluate the effects of PF4-triggered bone destruction in mice and determine the underlying mechanism. First, in
Quantitative profile of the uropathogenic Escherichia coli outer membrane proteome during growth in human urine
Alteri CJ and Mobley HLT
Infection and Immunity, 75(6), 2679-2688 (2007)
Yejun Tan et al.
Frontiers in immunology, 14, 1149810-1149810 (2023-04-11)
Patients with diabetes mellitus (DM) have a higher incidence of malignant tumors than people without diabetes, but the underlying molecular mechanisms are still unclear. To investigate the link between DM and cancer, we screened publicly available databases for diabetes and
25th Anniversary Article: Design of Polymethine Dyes for All-Optical Switching Applications: Guidance from Theoretical and Computational Studies
Gieseking RL, et al.
Advanced Materials, 26(1), 68-84 (2014)
Xiu-Qing Li et al.
Journal of virology, 97(6), e0041223-e0041223 (2023-05-31)
Pseudorabies virus (PRV) is a double-stranded DNA virus that causes Aujeszky's disease and is responsible for economic loss worldwide. Transmembrane protein 41B (TMEM41B) is a novel endoplasmic reticulum (ER)-localized regulator of autophagosome biogenesis and lipid mobilization; however, the role of
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