C3131
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
Synonyme(s) :
CYC, CytC
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Laissez-nous vous aiderNom du produit
Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
biological source
bovine heart
assay
≥95% based on Mol. Wt. 12,327 basis
form
powder
mol wt
12327 Da
storage condition
(Tightly closed Dry)
technique(s)
cell culture | mammalian: suitable
impurities
0.40-0.50% Iron (anhydrous)
solubility
water: 10 mg/mL, dark red-brown
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... CYC1(512500)
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Biochem/physiol Actions
Le passage rapide du cytochrome c entre les états ferreux et ferrique en font un transporteur d′électrons biologique efficace. Il joue un rôle essentiel dans les oxydations tant chez les plantes que chez les animaux. Il est généralement considéré comme un catalyseur universel de la respiration, car il forme un pont d′électrons essentiel entre les substrats respirables et l′oxygène.
L′interconversion rapide du cytochrome c entre les états ferreux et ferrique en font un transporteur d′électrons biologique efficace. Il joue un rôle essentiel dans les oxydations tant chez les plantes que chez les animaux. Il est généralement considéré comme un maillon essentiel de la chaîne respiratoire, car il forme un pont d′électrons essentiel entre les substrats respirables et l′oxygène.
Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis. It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C. Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space.
Other Notes
Pour en savoir plus sur le cytochrome c et le transport d′électrons, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
Application
Cytochrome C from bovine heart has been used:
- as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines
- to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells
- as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues
General description
Research area: Apoptosis
Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.
Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.
Preparation Note
Prepared with acetic acid without using TCA.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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