C7611
Monoclonal Anti-Caspase 12 antibody produced in rat
clone 14F7, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Anti-CASP-12, Anti-CASP12P1
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Laissez-nous vous aiderNom du produit
Monoclonal Anti-Caspase 12 antibody produced in rat, clone 14F7, purified immunoglobulin, buffered aqueous solution
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
14F7, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Casp12(12364)
rat ... Casp12(156117)
Application
Monoclonal Anti-Caspase 12 has been used in immunoblotting and immunocytochemistry (4% paraformaldehyde-fixed, paraffin-embedded).
Biochem/physiol Actions
Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.
Murine caspase 12 (CASP12) is activated during endoplasmic reticulum stress and activates other caspases which act on substrates, ultimately leading to apoptosis. The protein has been shown to be activated during progression of retinal degeneration in animal models. In mice models, CASP12 has been shown to cause neuronal death resulting from ischemia/reperfusion. Casp12 might stimulate nuclear factor-κB (NF-κB) by directing the post-translational degradation of inhibitor of kappa B (IκBα). Overexpression of caspase 12 induces apoptosis, which is inhibited by the pan caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethyl ketone (z-VAD-FMK).
Recognizes full length and possibly truncated p20 caspase 12.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.
Monoclonal Anti-Caspase 12 (rat IgG2a isotype) is derived from the 14F7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from CD rats immunized with a recombinant mouse caspase 12. Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspase 12 is ubiquitously expressed in mouse tissues, at high levels in muscle, liver and kidney and at moderate levels in brain, where it is present in cortical neurons, Purkinje cells, brainstem neurons, and olfactory neurons.
Immunogen
recombinant mouse caspase 12 (residues 95-318).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Activation of the unfolded protein response in infarcted mouse heart and hypoxic cultured cardiac myocytes
Thuerauf DJ, et al.
Circulation Research, 99(3), 275-282 (2006)
Chun-Yan Wang et al.
Molecular medicine reports, 16(3), 3587-3593 (2017-07-18)
It was previously confirmed that hydrogen sulfide (H2S) has a neuroprotective effect, preventing homocysteine‑induced neurotoxicity. However, the exact molecular mechanisms underlying this protective effect remain to be fully elucidated. Endoplasmic reticulum (ER) stress contributes to homocysteine‑induced neurotoxicity. Silent mating type
Activation of caspase-12 by endoplasmic reticulum stress induced by transient middle cerebral artery occlusion in mice
Shibata M, et al.
Neuroscience, Neuroscience.(2003;118), 2-2 (2003)
Caspase 12 degrades I kappaB alpha protein and enhances MMP-9 expression in human nasopharyngeal carcinoma cell invasion
Chu WK, et al.
Testing, 8(20), 33515-33515 (2017)
Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta
Nakagawa T, et al.
Nature, 403(6765), 98-98 (2000)
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