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Merck

C8487

Anti-Caspase 3, Active antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Activated Caspase 3 Antibody, Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit, Anti-Apopain, Anti-CPP32, Anti-Yama

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01321906
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
129

Principaux documents

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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, bovine, human, pig, canine, mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine, microarray: suitable, western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)

UniProt accession no.

P42574

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)

General description

Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.
Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.

Immunogen

Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.

Application

Anti-Caspase 3, Active antibody has been used:
  • for western blotting of cytochrome c for caspase activation
  • as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
  • in Western blot analysis of activated caspase 3
  • as a primary antibody in immunodetection of rat brain sections

Biochem/physiol Actions

Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000508965
0000475628
0000475628
0000445867
0000445867

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Consulter la Bibliothèque de documents

Caspases: an apoptosis mediator
Palai T K and Mishra S R
Journal of Advanced Veterinary and Animal Research, 2(1) (2015)
Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma
Liu PF, et al.
Testing, 12(7) (2017)
Paul Delgado-Olguín et al.
Development (Cambridge, England), 141(23), 4610-4617 (2014-11-02)
Maintenance of vascular integrity is required for embryogenesis and organ homeostasis. However, the gene expression programs that stabilize blood vessels are poorly understood. Here, we show that the histone methyltransferase Ezh2 maintains integrity of the developing vasculature by repressing a
Identification of SMURF1 as a possible target for 7q21. 3?22.1 amplification detected in a pancreatic cancer cell line by in?house array?based comparative genomic hybridization.
Suzuki A, et al.
Cancer Science, 99(5), 986-994 (2008)
Kalina Andreeva et al.
Ophthalmology and eye diseases, 6, 43-54 (2014-09-12)
Ischemia/reperfusion (IR) injury has been associated with several retinal pathologies, and a few genes/gene products have been linked to IR injury. However, the big picture of temporal changes, regarding the affected gene networks, pathways, and processes remains to be determined.
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Contenu apparenté

Data Sheet - C8487

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