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Merck

C9322

Catalase from bovine liver

lyophilized powder, 2,000-5,000 units/mg protein

Synonyme(s) :

H2O2:H2O2 oxidoreductase

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A propos de cet article

Numéro CAS:
9001-05-2
eCl@ss:
32160410
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-577-1
MDL number:
MFCD00081483
Numéro CE :
1.11.1.6 (BRENDA, IUBMB)
Specific activity:
2,000-5,000 units/mg protein
Biological source:
bovine liver

Principaux documents

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Nom du produit

Catalase from bovine liver, lyophilized powder, 2,000-5,000 units/mg protein

SMILES string

O(CC)C(=O)c1ccc(cc1)O

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

biological source

bovine liver

form

lyophilized powder

specific activity

2,000-5,000 units/mg protein

mol wt

tetramer ~250 kDa

isoelectric point

5.4

UniProt accession no.

P00432

shipped in

wet ice

storage temp.

−20°C

Quality Level

400

Gene Information

cow ... CAT(280743)

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Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

  • to prepare H2O2-O2 based biocathode for applications in glucose biofuel cells
  • to study the kinetic properties and storage stability of catalase immobilized on to florisil
  • in glutathione-mediated superoxide generation in an aqueous solution

Biochem/physiol Actions

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Disclaimer

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

General description

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Other Notes

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Preparation Note

Solutions of catalase should not be frozen. Freezing catalase solutions will lead to a loss of activity by 50-70%. The recommended storage temperature of the product in its powdered form is at -20 °C.
This product is a lyophilized powder with activity of 2,000-5,000 units/mg.
The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000522224
0000522224
0000517291
0000517291
0000376653

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Consulter la Bibliothèque de documents

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can Catalase from bovine liver, Product C9322, be reconstituted?

    This product is soluble in 50 mM potassium phosphate, pH 7.0 at a concentration of 10 mg/mL.

  4. How can a stock solution of Catalase from bovine liver, Product C9322, be stored?

    A 10 mg/mL stock solution prepared in 50 mM potassium phosphate, pH 7.0 and 50% glycerol was stable for at least 1 week in the freezer at -20°C.

  5. What salts are present in Catalase from bovine liver, Product C9322?

    Catalase (C9322) contains potassium phosphate buffered salts (and 20% trehalose as a stabilizer).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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James C Dooley et al.
Current biology : CB, 30(12), 2404-2410 (2020-05-16)
Cortical development is an activity-dependent process [1-3]. Regarding the role of activity in the developing somatosensory cortex, one persistent debate concerns the importance of sensory feedback from self-generated movements. Specifically, recent studies claim that cortical activity is generated intrinsically, independent
Julie Elizabeth Keeble et al.
Pain, 141(1-2), 135-142 (2008-12-09)
Inflammatory diseases associated with pain are often difficult to treat in the clinic due to insufficient understanding of the nociceptive pathways involved. Recently, there has been considerable interest in the role of reactive oxygen species (ROS) in inflammatory disease, but
Amol Aher et al.
Developmental cell, 46(1), 40-58 (2018-06-26)
The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening
Eduard N Lavrentyev et al.
American journal of physiology. Heart and circulatory physiology, 296(1), H106-H118 (2008-11-04)
In rat diabetic animal models, ANG(1-7) treatment prevents the development of cardiovascular complications. Angiotensin-converting enzyme (ACE)2 is a major ANG(1-7)-generating enzyme in vascular smooth muscle cells (VSMCs), and its expression is decreased by a prolonged exposure to high glucose (HG)
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