C9999
Citrate Buffer, pH 6.0, 10×, Antigen Retriever
for immunohistochemistry
Synonyme(s) :
1,2,3-Propanetricarboxylic acid, 2-hydroxy-, mixt. with sodium hydroxide (Na(OH)) and sodium 2-hydroxy-1,2,3-propanetricarboxylate
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A propos de cet article
NACRES:
NA.47
UNSPSC Code:
12352107
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Citrate Buffer, pH 6.0, 10×, Antigen Retriever, for immunohistochemistry
form
liquid
pH
6.0
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
Application
Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.
Other Notes
Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.
Preparation Note
Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.
Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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