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Merck

D2902

DMEM - low glucose

With ʟ-glutamine and sodium pyruvate, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonyme(s) :

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
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Nom du produit

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 10 g/L, clear

components

glucose: low
NaHCO3: no
phenol red: no
HEPES: no
L-glutamine: yes
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium - low glucose is used in cell line culture (used in medium for the growth of cells) and for their maintenance. It is also used for tissue culture.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Preparation Note

Formulated to contain 10.0 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.

pictograms

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signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Classe de stockage

11 - Combustible Solids


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The tridecamer d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to 13 nucleotides of the 3'- and 5'-reiterated terminal sequences of Rous sarcoma virus 35S RNA, was added to chick embryo fibroblast tissue cultures infected with Rous sarcoma virus. Inhibition of virus production resulted.
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D Solter et al.
Proceedings of the National Academy of Sciences of the United States of America, 75(11), 5565-5569 (1978-11-01)
A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described. This antibody, which reacts with embryonal carcinoma cells of mouse and human origin and with some preimplantation

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