Accéder au contenu
Merck

D8537

Solution saline dans un tampon phosphate de Dulbecco

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

DPBS

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Toutes les photos(1)

About This Item

UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75
MDL number:
MFCD00131855

Nom du produit

Solution saline dans un tampon phosphate de Dulbecco, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

Quality Level

300

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

La solution saline tamponnée au phosphate de Dulbecco (DPBS) a été utilisée :

  • pour la dilution de globules rouges frais afin d'étudier le piégeage d'agrégats de globules rouges.
  • pour laver les puits d'une plaque de microtitration.
  • pour laver des cellules avant de les remettre en suspension dans un tampon de lyse pour isoler de l'ADN.
  • comme constituant d'un milieu de conservation pour embryons destiné à la vitrification des embryons.

General description

La solution saline tamponnée au phosphate de Dulbecco (DPBS) est une solution saline équilibrée (BSS, Balanced Salt Solution) utilisée pour la manipulation et la culture des cellules de mammifère. Le tampon DPBS est utilisé pour irriguer, laver et diluer les cellules de mammifère tout en maintenant l'équilibre osmotique. Il apporte l'eau et les ions inorganiques essentiels au métabolisme normal des cellules. Le DPBS additionné de calcium et de magnésium facilite la liaison et l'agrégation cellulaires. Le PBS de Dulbecco sans ajout de ces ions peut être utilisé pour laver et rincer des cellules en suspension. Le tamponnage au phosphate maintient le pH dans la plage physiologique.

Other Notes

Lauréat du prix CiteAb 2024 récompensant les avancées des fournisseurs dans la recherche sur la maladie de Parkinson

Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000421766
0000421766
0000421784
0000421784
0000423710

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the formulation for Product D8537, Dulbecco's Phosphate?

    The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.    

  4. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

    This is a 1X concentration. The 10X concentration is listed under product D1408.

  5. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

    The pH will be in the range of 7.1-7.5, according to the specification sheet.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. What is a Salt Toxicity Test?

    The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted.  They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour.  The cells are counted and %viability determined.  The cells are then cultured for an additional 7 hours (total of 8 hours).  The cells are counted again and the % viability determined.  The viability at 1 hr has to be >95% of the original viability.  The viability at 8 hrs must be >75% of the original viability.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Cameron Goertzen et al.
Biological procedures online, 20, 20-20 (2018-10-26)
Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
Gensheng Wang et al.
Molecular therapy. Methods & clinical development, 3, 15052-15052 (2016-01-28)
Interleukin-1 (IL-1) plays an important role in the pathophysiology of osteoarthritis (OA), and gene transfer of IL-1 receptor antagonist (IL-1Ra) holds promise for OA treatment. A preclinical safety and biodistribution study evaluated a self-complementary adeno-associated viral vector carrying rat IL-1Ra
Afficher tous les articles scientifiques apparentés

Contenu apparenté

EZview Red Protein A and ANTI-FLAG M2 Affinity Gels

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001

Dulbecco Phosphate Buffered Saline - Formulation

Instructions

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique