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Merck

F2442

Sérum de veau fœtal

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonyme(s) :

FBS, FCS, sérum, sérums

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
MFCD00132239
biological source:
bovine fetus
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice

Nom du produit

Sérum de veau fœtal, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Quality Level

500

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Catégories apparentées

Analysis Note

  • A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
  • Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques

  • Taux d′endotoxines et d′hémoglobine testés

Application

Le sérum de veau fœtal entre également dans la composition des milieux et liquides suivants :
  • milieu de croissance de cellules hybrides mélanocyte/macrophage périvasculaire (PVM/M)
  • liquide tubaire de synthèse (SOF)
  • milieu essentiel minimum d'Eagle (EMEM)
  • milieu Neurobasal
  • milieu 5A de Hyclone McCoy
  • milieu de Hank

Biochem/physiol Actions

Le FBS reste un supplément pour milieux très prisé, car il assure de multiples fonctions en culture cellulaire. Il apporte des nutriments, des facteurs de croissance et d'adhésion et protège les cellules des dommages oxydatifs et de l'apoptose par des mécanismes qui sont difficiles à reproduire avec les milieux sans sérum.

General description

Le sérum de veau fœtal (SVF ou FBS en anglais) est un mélange complexe qui contient des molécules de bas et haut poids moléculaires et qui présente des fonctions optimales pour la stimulation et l'inhibition de la croissance. Le FBS contient des molécules comme des facteurs de croissance, des protéines, des vitamines, des oligoéléments, des hormones, etc. Ces molécules sont importantes pour la croissance et le maintien des cellules. C'est un supplément pour milieux de culture cellulaire d'usage courant. Le FBS est obtenu à partir de sang de veau. Il est dépourvu de fibrine et de facteurs de coagulation. Son inactivation par la chaleur inhibe le système du complément. Le FBS dérivé de sang coagulé est le supplément non défini le plus utilisé dans la culture des cellules eucaryotes et notamment des cellules de mammifères. Il est généralement utilisé avec les milieux classiques mis au point dans les années 1950 et 1960. Ces derniers comprennent de nombreux milieux très prisés, comme le milieu essentiel minimum (MEM), le milieu d'Eagle modifié par Dulbecco (DME ou DMEM), le milieu de Dulbecco modifié par Iscove (IMDM), le milieu M199 et le milieu RPMI 1640 (Roswell Park Memorial Institute).

Other Notes

Produit testé pour la présence de bactéries, de virus, de mycoplasmes et d'endotoxines et pour la teneur en hémoglobine. Produit filtré trois fois sur membrane de 0,1 µm en conditions aseptiques.

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
23N158-A
24B125
25A064
24E021
24B458

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

  1. What is the level of growth factors in Fetal Bovine Serum, Product F2442?

    Sigma Aldrich does not determine the levels of growth factors (such as TGF beta, VEGF, EGF, PDGF, or FGF) for serum with reduced levels of growth factors and plasma derived serum.

  2. Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?

    We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.

  3. How is Fetal Bovine Serum, Product F2442, cell culture tested?

    Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.

  4. What is the cloning assay performed on Fetal Bovine Serum, Product F2442?

    The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.

  5. What is the difference between Fetal Bovine Serum F2442 and 12103C?

    Product No. 12103C meets most of the specifications of F2442.  Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. Are all Fetal Bovine Serum (FBS) products heat inactivated?

    Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.

  8. There is a precipitate in my serum.  Is my serum contaminated?

    Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

  9. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. Does Sigma-Aldrich offer delipidated bovine serum?

    At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss

  13. Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?

    FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.

  14. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  15. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
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