F7378
Fluorescein diacetate
Powder
Synonyme(s) :
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
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About This Item
Nom du produit
Fluorescein diacetate, used as cell viability stain
InChI key
CHADEQDQBURGHL-UHFFFAOYSA-N
InChI
1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
form
powder
technique(s)
titration: suitable
color
white to faint yellow
mp
200-203 °C (lit.)
solubility
acetone: 25 mg/mL
ε (extinction coefficient)
≥86000 at 488-494 nm in 0.1 M NaOH
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Catégories apparentées
Application
General description
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Is Product F7378, Fluorescein diacetate, a fluorescent compound?
No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein. Only the fluorescein form is fluorescent.
After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?
The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.
What are some common applications for Product F7378, Fluorescein diacetate?
This compound is used extensively for the staining of living cells. It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964."
What solubility information do you have on Product F7378, Fluorescein diacetate?
We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).
How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?
Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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