G2N350
GenElute™ Plant Genomic DNA Miniprep Kit
sufficient for 350 purifications
Synonyme(s) :
Plant Genomic DNA Miniprep, Gen Elute
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NACRES:
NA.55
UNSPSC Code:
41105501
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sufficient for 350 purifications
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
Quality Level
input
plant tissue
test parameters
sample volume: 100 mg
greener alternative category
, Aligned
storage temp.
15-25°C
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Catégories apparentées
General description
The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
The GenElute Plant Genomic DNA Purification Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of plant species. This kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Biochem/physiol Actions
Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
Features and Benefits
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Réf. du produit
Description
FDS
- C2112Column Preparation Solution
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C
supp_hazards
Classe de stockage
8A - Combustible corrosive hazardous materials
wgk
WGK 2
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V Roussel et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 111(1), 162-170 (2005-05-12)
A sample of 480 bread wheat varieties originating from 15 European geographical areas and released from 1840 to 2000 were analysed with a set of 39 microsatellite markers. The total number of alleles ranged from 4 to 40, with an
Daniel Lunn et al.
Plant physiology and biochemistry : PPB, 66, 91-97 (2013-03-19)
Fruit development entails a multitude of biochemical changes leading up to the mature green stage. During this period the cell wall will undergo complex compositional and structural changes. Inhibition of genes encoding elements of the machinery involved in trafficking to
Catherine Ravel et al.
Genome, 49(9), 1131-1139 (2006-11-18)
Information on single-nucleotide polymorphisms (SNPs) in hexaploid bread wheat is still scarce. The goal of this study was to detect SNPs in wheat and examine their frequency. Twenty-six bread wheat lines from different origins worldwide were used. Specific PCR-products were
Jochen C Reif et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 111(5), 838-845 (2005-07-22)
It has been claimed that the system that delivers the products of plant breeding reduces the diversity of cultivated varieties leading to an increased genetic vulnerability. The main goal of our study was to monitor the temporal trends in genetic
Catherine Ravel et al.
Functional & integrative genomics, 6(4), 310-321 (2006-03-29)
Wheat prolamin-box binding factor (WPBF) was shown to be an activator of Triticum aestivum L. storage protein genes. Three homoeologous genes encoding this transcription factor were isolated from a bacterial artificial chromosome genomic library and sequenced. The genes all have
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