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Merck

H0163

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal

clone HDAC4-144, purified from hybridoma cell culture

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD05665442
Conjugate:
unconjugated
Clone:
HDAC4-144, monoclonal
Application:
ICC, IP, WB
Species reactivity:
human, rat, mouse
Citations:
3
Technique(s):
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells
Uniprot accession no.:
P56524

Principaux documents

Voir toute la documentation
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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HDAC4-144, monoclonal

form

buffered aqueous solution

mol wt

antigen ~140 kDa

species reactivity

human, rat, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells

isotype

IgG2a

UniProt accession no.

P56524

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)

Catégories apparentées

Application

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation

Biochem/physiol Actions

Histone deacetylase (HDAC) catalyzes the deacetylation of histones. HDAC4 is implicated in transcriptional repression. It dynamically migrates between nucleus and cytoplasm through its nuclear import and export signals. Interaction of HDAC4 with 14-3-3 and myocyte enhancer factor-2 (Mef2) proteins disturbs such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Histone Deacetylase 4 (HDAC4) (mouse IgG2a isotype) is derived from the HDAC4-144 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human HDAC4 with C-terminal added lysine, conjugated to KLH. HDAC4 belongs to the class II of Mammalian HDACs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Classe de stockage

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000214434
0000090208
0000090208
0000214434
0000107888

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Histone deacetylase 4 possesses intrinsic nuclear import and export signals
Wang AH, et al.
Molecular and Cellular Biology, 21, 5992-6005 (2001)
Erasers of Histone Acetylation: The Histone Deacetylase Enzymes
Seto E, et al.
Cold Spring Harbor Perspectives in Biology, 6 (2014)
Toon Verheyen et al.
Nucleic acids research, 43(12), 5771-5784 (2015-05-21)
Protein phosphatase-1 (PP1) is a key regulator of transcription and is targeted to promoter regions via associated proteins. However, the chromatin binding sites of PP1 have never been studied in a systematic and genome-wide manner. Methylation-based DamID profiling in HeLa

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