H2387
Hanks′ Balanced Salts
Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
HBSS
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powder
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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Catégories apparentées
General description
Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.
Application
Hanks′ Balanced Salt has been used:
- to culture primary hippocampal neuron cells from rats
- to collect the rat brains
- to culture rat primary hepatocytes
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
Other Notes
The pH is adjusted after addition of sodium bicarbonate.
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Abdelrahman I Rezk et al.
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Seo Yeon Lee et al.
Polymers, 12(2) (2020-02-20)
Herein, we describe precisely a covalent modification of pure magnesium (Mg) surface and its application to induce in vitro osteogenic differentiation. The new concept of a chemical bonding method is proposed for developing stable chemical bonds on the Mg surface
Bikendra Maharjan et al.
Materials science & engineering. C, Materials for biological applications, 114, 111056-111056 (2020-10-01)
Despite intensive attempts to fabricate polypyrrole nanoparticles (PPy-NPs) incorporated nanofibrous scaffolds, a low-cost facile strategy is still demanded. Herein, we developed a novel strategy- in-situ polymerization of PPy-NPs and immobilized them into the PCL polymeric matrix in a single step.
Mostafa M H Ibrahim et al.
ASN neuro, 12, 1759091420974134-1759091420974134 (2020-11-13)
Norepinephrine (NE) control of hypothalamic gluco-regulation involves astrocyte-derived energy fuel supply. In male rats, exogenous NE regulates astrocyte glycogen metabolic enzyme expression in vivo through 5'-AMP-activated protein kinase (AMPK)-dependent mechanisms. Current research utilized a rat hypothalamic astrocyte primary culture model
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