H5505
Histone from calf thymus
Type III-S
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
About This Item
Numéro CAS:
UNSPSC Code:
41106305
NACRES:
NA.51
Beilstein/REAXYS Number:
1895465
MDL number:
Nom du produit
Histone from calf thymus, Type III-S
biological source
bovine (calf) thymus
type
Type III-S
form
powder
concentration
≥60% protein (biuret)
technique(s)
activity assay: suitable
solubility
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
storage temp.
2-8°C
Quality Level
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Application
Histone is suitable for use:
- as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
- in Cdk5 activity assay
- in histone H1 kinase assay
- as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
- as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells
Biochem/physiol Actions
Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.
Features and Benefits
Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.
Other Notes
Characterized as mainly subgroup f1 by SDS-PAGE
Preparation Note
Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Mária Čížková et al.
Cells, 8(7) (2019-07-20)
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter
P Balsas et al.
Journal of hematology & oncology, 10(1), 80-80 (2017-04-01)
Pharmacological inhibition of B cell receptor (BCR) signaling has recently emerged as an effective approach in a wide range of B lymphoid neoplasms. However, despite promising clinical activity of the first Bruton's kinase (Btk) and spleen tyrosine kinase (Syk) inhibitors
Amit K Dutta et al.
Journal of colloid and interface science, 324(1-2), 55-60 (2008-05-30)
The real-time changes in viscoelasticity of adsorbed poly(L-lysine) (PLL) and adsorbed histone (lysine rich fraction) due to cross-linking by glutaraldehyde and corresponding release of associated water were investigated using a quartz crystal microbalance with dissipation monitoring (QCM-D) and attenuated total
Scott T
Concise encyclopedia of biochemistry, 265-265 (1988)
Liang Zheng et al.
Haematologica, 105(4), 1107-1119 (2019-11-23)
Thrombotic thrombocytopenic purpura (TTP) is caused by severe deficiency of ADAMTS13 (A13), a plasma metalloprotease that cleaves endothelium-derived von Willebrand factor (VWF). However, severe A13 deficiency alone is often not sufficient to cause an acute TTP; additional factors may be
Contenu apparenté
Instructions
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique