H6512
Heparinase II from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100 units/mg protein (enzyme + BSA)
Synonyme(s) :
Heparin lyase II
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A propos de cet article
Specific activity:
≥100 units/mg protein (enzyme + BSA)
Biological source:
bacterial (Flavobacterium heparinum)
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Laissez-nous vous aiderbiological source
bacterial (Flavobacterium heparinum)
conjugate
conjugate (Glucosaminoglycan)
form
lyophilized powder
specific activity
≥100 units/mg protein (enzyme + BSA)
mol wt
84.1 kDa
storage temp.
−20°C
Quality Level
General description
Heparinase II is one of three key heparin-degrading enzymes of Flavobacterium heparinum (also known as Pedobacter heparinus1). It belongs to the polysaccharide lyases family PL21.
Application
Heparinase II from Flavobacterium heparinum has been used:
- for digestion of heparin sulfate during exosome isolation
- for digestion of heparin sulfate in notochordal cell conditioned media (NCCM) to investigate the content of glycosaminoglycans in NCCM
- as a component of digestion buffer during cell surface glycan processing
- as a component of heparin lyase (HSase) mix to remove the heparin sulfate on the 293ͰT/ACE2 cell surface and study the effect of histones on the infectivity of pseudovirus
Biochem/physiol Actions
Heparinase II cleaves heparan sulfate, and to a lesser extent heparin (relative activity about 2:1), at the α (1-4) linkages between hexosamines and uronic acid residues (both glucuronic and iduronic), yielding mainly disaccharides. Heparinase II has the broadest substrate specificity of the three heparinases Heparinase enzymes helps in decoding the complex structures of substrates. Heparin interferes with DNA transcription in PCR and in reverse transcription of RNA. Heparinase II has been used to remove heparin for downstream analysis of genomic DNA.
Other Notes
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.0 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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McLean, M.W., et al.
Proc. 8th Int. Symp. Glycoconjugates, 1, 73-73 (1985)
PCR-sequence-specific primer typing of HLA class I and class II alleles.
Mike Bunce
Methods in molecular biology (Clifton, N.J.), 210, 143-171 (2002-11-05)
Moffat, C.F., et al.
Proc. 8th Int. Symp. Glycoconjugates, 1, 79-79 (1985)
David Shaya et al.
The Journal of biological chemistry, 285(26), 20051-20061 (2010-04-21)
Heparinase II (HepII) is an 85-kDa dimeric enzyme that depolymerizes both heparin and heparan sulfate glycosaminoglycans through a beta-elimination mechanism. Recently, we determined the crystal structure of HepII from Pedobacter heparinus (previously known as Flavobacterium heparinum) in complex with a
Devina Purmessur et al.
The spine journal : official journal of the North American Spine Society, 15(5), 1060-1069 (2015-02-11)
Painful human intervertebral discs (IVDs) exhibit nerve growth deep into the IVD. Current treatments for discogenic back pain do not address the underlying mechanisms propagating pain and are often highly invasive or only offer temporary symptom relief. The notochord produces
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