H6533
Anticorps monoclonal Anti-HA
clone HA-7, purified from hybridoma cell culture
Synonyme(s) :
Anti-HA, Anti-hémagglutinine de la grippe
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A propos de cet article
Conjugate:
peroxidase conjugate
Clone:
HA-7, monoclonal
Application:
WB
Citations:
135
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mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent
storage temp.
2-8°C
General description
Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.
The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.
Immunogen
Peptide synthétique correspondant à un fragment de l′hémagglutinine (HA) du virus de la grippe humain, connu sous le nom d′étiquette HA ou HA-tag, conjugué à KLH.
Application
Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:
- in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells
- in immunoblot detection in transfected A375 cells
- is suitable for immunoblotting of nucleosome assembly protein NAP1L1
Biochem/physiol Actions
L′anticorps reconnaît tant les formes natives que dénaturées et réduites des protéines de fusion avec une étiquette HA. Il réagit avec les protéines de fusion exprimées dans E. coli ou dans les cellules de mammifères et dont l′étiquette HA se situe à l′extrémité N-terminale ou C-terminale.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Reconstitute in .5mL of distilled water.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Joanna F McGouran et al.
Chemistry & biology, 20(12), 1447-1455 (2013-12-03)
Posttranslational modification with ubiquitin (Ub) controls many cellular processes, and aberrant ubiquitination can contribute to cancer, immunopathology, and neurodegeneration. The versatility arises from the ability of Ub to form polymer chains with eight distinct linkages via lysine side chains and
Ernest C So et al.
The Journal of biological chemistry, 291(11), 5832-5843 (2016-01-13)
Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the
Hepatitis C virus NS5A targets nucleosome assembly protein NAP1L1 to control the innate cellular response
Cevik RE, et al.
Journal of virology, 91(18), e00880-e00817 (2017)