H8162
HIS-Select® Cobalt Affinity Gel
(1:1 suspension in a 30% ethanol solution)
Synonyme(s) :
cobalt affinity gel, cobalt charged agarose
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A propos de cet article
NACRES:
NA.56
UNSPSC Code:
41106500
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(1:1 suspension in a 30% ethanol solution)
shelf life
1 yr (when stored properly.)
technique(s)
protein purification: suitable
Quality Level
matrix
6% Beaded Agarose
capacity
>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)
storage temp.
2-8°C
General description
The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.
Application
HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select® Cobalt Affinity Gel has been used to purify His-tagged proteins.
Physical form
1:1 suspension in a 30% ethanol solution
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
wgk
WGK 3
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Classe de stockage
3 - Flammable liquids
flash_point_f
118.4 °F - closed cup
flash_point_c
48 °C - closed cup
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