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Merck

HPA002689

Anti-WASHC1 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonyme(s) :

Anti-FAM39E, Anti-FLJ00038, Anti-WASH1

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.43
Human Protein Atlas Number:
HPA002689
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC
Citations:
14

Principaux documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

enhanced validation

RNAi knockdown
Learn more about Antibody Enhanced Validation

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:1000-1:2500

immunogen sequence

GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... WASH1(100287171)

General description

WASH complex subunit 1 (WASHC1) is located on human chromosome 9p24.3. It belongs to Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) family. WASHC1 protein has an actin binding domain.

Immunogen

WASH complex subunit 1 recombinant protein epitope signature tag (PrEST)

Application

Anti-WASHC1 antibody produced in rabbit has been used:
  • in the detection of WASH complex in Dictyostelium cells by immunofluorescence imaging.
  • in the detection of WASH proteins in HeLa cells using confocal microscopy.
  • in western blotting for the detection of WASH1 complexes in human leukemic Jurkat T cells.

Biochem/physiol Actions

WASH exists as multiple complex protein in HeLa and bovine cells. WASH proteins coordinate the migration of endosomal α5β1 integrin. WASHC1 protein regulates actin cytoskeleton organisation. WASH1 protein is regarded as actin-nucleating promoting factor. WASH protein complex interacts with actin related protein, Arp2/3 and mediates actin networking.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST74318

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
A33116
R00479
R000479

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics
Harbour ME, et al.
The Journal of Biological Chemistry, jcs-071472 (2010)
WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors
Buckley CM, et al.
Proceedings of the National Academy of Sciences of the USA, 113(40), E5906-E5915 (2016)
The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex
Derivery E, et al.
Developmental Cell, 17(5), 712-723 (2009)
Human subtelomeric WASH genes encode a new subclass of the WASP family
Linardopoulou EV, et al.
PLoS Genetics, 3(12), e237-e237 (2007)
The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated invasive migration
Zech T, et al.
Journal of Cell Science, 124(22), 3753-3759 (2011)
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