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Merck

I1146

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

Human IGF-1, IGF-1 Protein, Recombinant IGF-1

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A propos de cet article

NACRES:
NA.77
UNSPSC Code:
51111800
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥85% (HPLC)
Form:
lyophilized powder
Mol wt:
7,676 Da

Principaux documents

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Nom du produit

R3 IGF-1 human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥85% (HPLC)

form

lyophilized powder

potency

≤10 ng/mL ED50

quality

endotoxin tested

mol wt

7,676 Da

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P05019

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... IGF1(3479)

Catégories apparentées

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Biochem/physiol Actions

Proliferation of chinese hamster ovary CHO cells.

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Preparation Note

Prepared from 0.1 M acetic acid

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000446231
0000446231
0000422248
0000422248
0000352239

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?

    The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.

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