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Merck

L3001

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg solid

Synonyme(s) :

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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A propos de cet article

Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
Numéro CE :
MDL number:
Specific activity:
5-15 units/mg solid
Biological source:
wheat (germ)
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biological source

wheat (germ)

Quality Level

type

Type I

form

lyophilized powder

specific activity

5-15 units/mg solid

UniProt accession no.

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Gene Information

wheat ... lipase(543006)

General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:
  • to prepare enzyme solution for the biodegradability test
  • to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
  • to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Analysis Note

Contains acid phosphatase.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Contenu apparenté


Ondřej Dlouhý et al.
Cells, 10(9) (2021-09-29)
Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Preparation and characterization of poly (ethylene glycol)-crosslinked reacetylated chitosans
Dal P A, et al.
Carbohydrate Polymers, 42(2), 201-206 (2000)