L5283
Leukemia Inhibitory Factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Synonyme(s) :
Human LIF, Human Leukemia Inhibitory Factor, hLIF, LIF, rLIF
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Laissez-nous vous aiderNom du produit
Leukemia Inhibitory Factor human, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
buffered aqueous solution (pH 7.4)
potency
0.1-1.5 ng/mL ED50/EC50
quality
endotoxin tested
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
Quality Level
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Analysis Note
The proliferative activity of human LIF is measured in culture using the human leukemic cell line, TF-1.
Biochem/physiol Actions
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Physical form
Solution, 0.2 μm filtered, in phosphate buffered saline containing 0.02% TWEEN® 20.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Proceedings of the National Academy of Sciences of the United States of America, 85(8), 2623-2627 (1988-04-01)
A human homologue of the recently cloned murine leukemia-inhibitory factor (LIF) gene was isolated from a genomic library by using the murine cDNA as a hybridization probe. The nucleotide sequence of the human gene indicated that human LIF has 78%
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