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Merck

L9655

Linoleic Acid-Oleic Acid-Albumin, 100x

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

Fatty Acid-Albumin-complexes

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352211

Nom du produit

Linoleic Acid-Oleic Acid-Albumin, 100x, liquid, sterile-filtered, BioReagent, suitable for cell culture

biological source

protein from bovine

sterility

sterile-filtered

product line

BioReagent

form

liquid

composition

linoleic acid and oleic acid, 2 mol/mol albumin (each)

concentration

100 mg/mL BSA in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Quality Level

300

Application

Linoleic Acid-Oleic Acid-Albumin, 100x has been used:
  • to induce acute hepatocellular stress in primary hepatocytes
  • to induce oxidative stress in cytotrophoblasts
  • to induce lipid droplet accumulation in HepG2 cells

General description

Fatty Acid-Albumin-complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) is beneficial to the growth of a variety of cell types (e.g., baby hamster kidney (BHK) hybridoma). A mixture of poly-unsaturated and monosaturated fatty acids (i.e., linoleic acid and oleic acid) produces synergistic effect when used as a media supplement.

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000315835
SLCM6796
0000352580
0000352578
0000352578

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Laura Moody et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(2), 141-152 (2019-01-04)
Carnitine palmitoyltransferase 1 (Cpt1a) is a rate-limiting enzyme that mediates the transport of fatty acids into the mitochondria for subsequent beta-oxidation. The objective of this study was to uncover how diet mediates the transcriptional regulation of Cpt1a. Pregnant Sprague Dawley
Pierluigi Ramadori et al.
Free radical biology & medicine, 91, 114-126 (2015-12-25)
Generation of reactive oxygen species (ROS) in response to fatty acids accumulation has been classically proposed as a possible "second hit" triggering progression from simple steatosis to non-alcoholic steatohepatitis (NASH). In this study we challenged hepatocyte-specific Keap1 knockout mice (Keap1(Δhepa))
Anna Virginia Adriana Pirozzi et al.
Antioxidants (Basel, Switzerland), 10(1) (2021-01-06)
Several plant extracts are acquiring increasing value because of their antioxidant activity and hypolipidemic properties. Among them, great interest has been recently paid to açai fruit as a functional food. The aim of this study was to test the ability
Jan Frohlich et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(6), 158920-158920 (2021-03-09)
Hepatocellular carcinoma (HCC) is one of the fastest-growing causes of cancer-related mortalities worldwide and this trend is mimicked by the surge of non-alcoholic fatty liver disease (NAFLD). Altered hepatic lipid metabolism promotes HCC development through inflammation and activation of oncogenes.
Yukimasa Takeda et al.
Scientific reports, 10(1), 3775-3775 (2020-03-01)
Brown adipocytes coordinate systemic energy metabolism associated with the pathogenesis of obesity and related metabolic diseases including type 2 diabetes. We have previously reported chemical compound-induced brown adipocytes (ciBAs) converted from human dermal fibroblasts without using transgenes. In this study
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