LYSISO1
Lysosome Isolation Kit
sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells
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A propos de cet article
UNSPSC Code:
12352200
EC Number:
233-140-8
NACRES:
NA.32
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Laissez-nous vous aiderNom du produit
Lysosome Isolation Kit, sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells
usage
sufficient for 20 mL (packed cells)
sufficient for 25 g (tissue)
technique(s)
centrifugation: suitable
fractionation: suitable
storage temp.
2-8°C
Quality Level
Application
Lysosome Isolation Kit has been used:
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.
- in the isolation of cytosol and lysosome
- to obtain enriched light and heavy lysosomal fractions from cultured cells
- in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
- in lysosome purification
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.
Features and Benefits
- Generates functional organelles for metabolism and disease research
- Includes protease inhibitor to manage degradation
- Measures integrity with included Neutral Red dye
- Choose from multiple options to obtain enriched fractions
- Enriched fractions for intact and functional lysosomes from tissues and cells
- Suitable for functional studies including protein degradation
General description
Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Classe de stockage
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections
Xu B, et al.
Molecular Immunology, 87(1), 122-131 (2017)
Fang-Shin Nian et al.
Molecular neurobiology, 56(9), 6095-6105 (2019-02-06)
Mutations in RAB18, a member of small G protein, cause Warburg micro syndrome (WARBM), whose clinical features include vision impairment, postnatal microcephaly, and lower limb spasticity. Previously, our Rab18-/- mice exhibited hind limb weakness and spasticity as well as signs
Xiaowei Ma et al.
ACS nano, 5(11), 8629-8639 (2011-10-07)
Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. Autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. Previous studies have shown that nanoparticles from various sources
Facilitated mitochondrial import of anti-viral and anti-cancer nucleoside drugs by human equilibrative nucleoside transporter-3 (hENT3)
Govindarajan R, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology (2009)
Activation of Nlrp3 inflammasomes enhances macrophage lipid-deposition and migration: implication of a novel role of inflammasome in atherogenesis
Li X, et al.
PLoS ONE, 9(1), e87552-e87552 (2014)
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