M0644
Milieu essentiel minimum d′Eagle
Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
MEM
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NACRES:
NA.75
UNSPSC Code:
12352207
Service technique
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form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
Application
Minimum essential medium Eagle has been used to:
- maintain natural killer (NK) cell lines
- culture Leishmania donovani strains
- culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles
Preparation Note
Formulated to contain 10.1 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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