M3024
Milieu essentiel minimum d′Eagle
with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
Synonyme(s) :
MEM
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12352207
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powder
technique(s)
cell culture | mammalian: suitable
components
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Catégories apparentées
General description
Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used:
- to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
- to culture MCF-7 cells for experimental treatments with hormone
- to maintain zebrafish pineal glands for action spectrum analysis
Features and Benefits
Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells
Preparation Note
Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Uterine leiomyomas or fibroids (UFs) are benign tumors characterized by hyperplastic smooth muscle cells and excessive deposition of extracellular matrix (ECM). Afflicting ~80% of women, and symptomatic in 25%, UFs bring tremendous suffering and are an economic burden worldwide; they
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