IgM, Kappa from murine myeloma

IgM, Kappa from murine myeloma has been used in:

• sandwich enzyme linked immunosorbent assay (ELISA)
• flow cytometry
• dot-immunobinding assay

IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid. IgM linked agarose resins have been tested for efficient conjugate binding.

The TEPC 183 tumor line is pristine-induced plasmacytoma-originated and carried subcutaneously in BALB/c mice. The hapten binding specificity of the TEPC 183 line has not been determined. It specifically recognizes mouse IgM. Identity and purity of the immunoglobulin is established by immunoelectrophoresis.

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Determined by the absorbance at 280 nm (E^1%/₂₈₀ = 11.8). Each vial contains at least 1 mg of purified myeloma protein.

IgM antibodies are present as pentamers in the serum and are produced in response to antigens IgM, κ from murine myeloma is produced by TEPC 183 tumor line introduced subcutaneously in BALB/c mice.

IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It is secreted by peritoneal B cells.

Solution in 0.05 M Tris, 0.5 M sodium chloride, pH 8.0, containing 0.02% sodium azide