M5650
Milieu essentiel minimum d’Eagle
With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
MEM
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UNSPSC Code:
12352207
NACRES:
NA.75
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Milieu essentiel minimum d′Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used to culture:
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
- HeLa cells
- Madin-Darby canine kidney (MDCK) cells
- chondrosarcoma cell line
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
Preparation Note
Supplement with 0.292 g/L L-glutamine.
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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