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Merck

M6818

Anti-MeCP2 antibody, Mouse monoclonal

clone Mec-168, purified from hybridoma cell culture

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02686372
Conjugate:
unconjugated
Clone:
Mec-168, monoclonal
Application:
immunocytochemistry
indirect ELISA
microarray
western blot
Species reactivity:
rat, human, mouse
Citations:
26
Technique(s):
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
Uniprot accession no.:
P51608

Principaux documents

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Nom du produit

Anti-MeCP2 antibody, Mouse monoclonal, clone Mec-168, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Mec-168, monoclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.

isotype

IgG1

UniProt accession no.

P51608

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)

Catégories apparentées

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunostaining
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)

Biochem/physiol Actions

Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).

Immunogen

synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.

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Classe de stockage

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000490139
0000490139
0000458273
0000458274
0000458273

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Consulter la Bibliothèque de documents

Xiangling Meng et al.
eLife, 5 (2016-06-22)
Many postnatal onset neurological disorders such as autism spectrum disorders (ASDs) and intellectual disability are thought to arise largely from disruption of excitatory/inhibitory homeostasis. Although mouse models of Rett syndrome (RTT), a postnatal neurological disorder caused by loss-of-function mutations in
The molecular basis of variable phenotypic severity among common missense mutations causing Rett syndrome
Brown K, et al.
Human Molecular Genetics, 25(3), 558-570 (2015)
Unexpected X Chromosome Skewing during Culture and Reprogramming of Human Somatic Cells Can Be Alleviated by Exogenous Telomerase
Pomp, Oz and others
Cell Stem Cell, 9(2), 156-165 (2011)
The biological functions of the methyl-CpG-binding protein MeCP2 and its implication in Rett syndrome
Nan X, et al.
Brain & Development, 23, S32-S37 (2001)
Rett syndrome mutations abolish the interaction of MeCP2 with the NCoR/SMRT co-repressor
Lyst MJ, et al.
Nature Neuroscience, 16(7) (2013)
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