M7292
M16 Medium
With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
Synonyme(s) :
Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12352207
Service technique
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Laissez-nous vous aiderQuality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
Application
M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.
Preparation Note
Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.
Analysis Note
Presence of particulates in solution will not affect product′s performance.
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
Classe de stockage
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Neil Cw Mackenzie et al.
Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research, 6(2), 416-421 (2011-02-26)
Transgenic technologies can provide important animal models for studying drug-metabolizing enzymes. Our overall aim was to generate versatile cell and animal systems that exhibited varying levels of cytochrome P450 oxidoreductase (POR) activity, more accurately modelling the human population for pharmacological
Xinyan Cao et al.
International journal of molecular sciences, 20(9) (2019-05-01)
Embryo implantation in the mink follows the pattern of many carnivores, in that preimplantation embryo diapause occurs in every gestation. Details of the gene expression and regulatory networks that terminate embryo diapause remain poorly understood. Illumina RNA-Seq was used to