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Merck

M7802

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.44
MDL number:
MFCD01633612
Conjugate:
unconjugated
Clone:
ERK-PT115, monoclonal
Application:
capture ELISA
immunocytochemistry
microarray
western blot
Species reactivity:
rat, human
Citations:
16
Technique(s):
capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol
Uniprot accession no.:
P28482

Principaux documents

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Nom du produit

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse, ~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ERK-PT115, monoclonal

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

species reactivity

rat, human

concentration

~2 mg/mL

technique(s)

capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol

isotype

IgG1

UniProt accession no.

P28482

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)

Application

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
  • immunoblotting
  • western blotting
  • immunofluorescence
  • enzyme linked immunosorbent assay (ELISA)
  • immunochemistry

Biochem/physiol Actions

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr183 in ERK-2).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Immunogen

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Classe de stockage

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000207702
0000179089
0000207702
0000179089
038M4863V

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Consulter la Bibliothèque de documents

A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma
Kondapalli KC, et al.
Nature Communications, 6(5), 6289-6289 (2015)
Transcriptional consequences of 16p11. 2 deletion and duplication in mouse cortex and multiplex autism families
Blumenthal I, et al.
American Journal of Human Genetics, 94(6), 870-883 (2014)
Evaluation of the role of MAPK1 and CREB1 polymorphisms on treatment resistance, response and remission in mood disorder patients
Calati R, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 44, 271-278 (2013)
Frances Evans et al.
BioMed research international, 2021, 2641068-2641068 (2021-11-02)
Gramicidin is a thoroughly studied cation ionophore widely used to experimentally manipulate the plasma membrane potential (PMP). In addition, it has been established that the drug, due to its hydrophobic nature, is capable of affecting the organization of membrane lipids.
Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis
Huang YWA, et al.
Cell, 148(5), 933-946 (2012)
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