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Merck

MAK126

Bilirubin Assay Kit

sufficient for 180 colorimetric tests

Synonyme(s) :

Hematoidin Assay Kit

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A propos de cet article

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 180 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder; gastrointestinal diseases

storage temp.

2-8°C

General description

Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.

Application

Bilirubin Assay Kit has been used to measure the total bilirubin concentration in samples.
Suitable for the detection of total and conjugated bilirubin in serum samples.
Not suitable for plasma samples. Anticoagulants in plasma interfere with the assay

Biochem/physiol Actions

The assay, based on the improved Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin. Total bilirubin is assessed using caffeine benzoate to split bilirubin from the unconjugated bilirubin protein complex.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Consulter la Bibliothèque de documents

Ji-Yoon Kim et al.
BMB reports, 53(3), 148-153 (2019-08-14)
Erythropoietin and iron have individually shown beneficial effects on early-phase liver regeneration following partial hepatectomy (PHx); however, there are limited data on the combined effect on late-phase liver regeneration after PHx. Here we examined combined effects of recombinant human erythropoietin
Morgane M Thibaut et al.
Journal of cachexia, sarcopenia and muscle, 12(1), 70-90 (2020-12-23)
Cancer cachexia is a debilitating metabolic syndrome contributing to cancer death. Organs other than the muscle may contribute to the pathogenesis of cancer cachexia. This work explores new mechanisms underlying hepatic alterations in cancer cachexia. We used transcriptomics to reveal
Kimberly J Jasmer et al.
Pigment cell & melanoma research, 33(6), 850-868 (2020-06-20)
Biosynthesis and degradation of heme, an iron-bound protoporphyrin molecule utilized by a wide variety of metabolic processes, are tightly regulated. Two closely related enzymes, heme oxygenase 1 (HMOX1) and heme oxygenase 2 (HMOX2), degrade free heme to produce carbon monoxide
Zhi-Ming Ding et al.
Frontiers in pharmacology, 9, 410-410 (2018-05-17)
Understanding of the temporal changes of hepatic lesions in the progression and regression of non-alcoholic steatohepatitis (NASH) is vital to elucidation of the pathogenesis of NASH, and critical to the development of a strategy for NASH pharmacotherapy. There are challenges
Bin Dong et al.
Journal of lipid research, 58(2), 350-363 (2016-12-13)
The farnesoid X receptor (FXR) plays critical roles in plasma cholesterol metabolism, in particular HDL-cholesterol (HDL-C) homeostasis. Obeticholic acid (OCA) is a FXR agonist being developed for treating various chronic liver diseases. Previous studies reported inconsistent effects of OCA on

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