N0142
Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse
clone N52, ascites fluid
Synonyme(s) :
Anti-CMT2CC, Anti-NFH
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A propos de cet article
Conjugate:
unconjugated
Clone:
N52, monoclonal
Application:
ARR, IHC (f), IHC (p), WB
Citations:
311
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Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
N52, monoclonal
contains
15 mM sodium azide
species reactivity
wide range
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): 1:400 using rat cerebellum, microarray: suitable, western blot: 1:1,000 using rat brain extract
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)
General description
Neurofilaments are composed of three subunits, namely NEFL (light), NEFM (medium) and NEFH (heavy). NEFH (neurofilament heavy) gene encodes the most important neuron-specific intermediate filament of cytoskeleton in myelinated axons. This gene is mapped to human chromosome 22q12.2.
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
C-terminal segment of enzymatically dephosphorylated pig neurofilament 200.
Application
Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody has been used in immunohistochemistry and western blotting.
Biochem/physiol Actions
Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques.
The heavy polypeptide neurofilament encoded by neurofilament heavy chain (NEFH) gene participates in the maintenance of cytoskeleton and axonal architecture in the proximal axonal region of spinal motoneurons. Mutations in the NEFH gene has a key role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). NEFH gene is also identified to generate autosomal dominant axonal Charcot-Marie-tooth disease (CMT2cc).
Physical form
Supplied as ascites fluid containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death.
Jacquier A< et al.
Acta Neuropathologica Communications, 5(1), 55-55 (2017)
Fibrin/Schwann cell matrix in poly-epsilon-caprolactone conduits enhances guided nerve regeneration.
Galla TJ, et al.
The International Journal of Artificial Organs, 27(2), 127-136 (2004)
Clinical and genetic basis of familial amyotrophic lateral sclerosis.
Souza PVSD, et al.
Arquivos de Neuro-Psiquiatria, 73(12), 1026-1037 (2015)
Yoshifumi Ueta et al.
Frontiers in neural circuits, 7, 164-164 (2013-10-19)
The frontal cortex plays an important role in the initiation and execution of movements via widespread projections to various cortical and subcortical areas. Layer 2/3 (L2/3) pyramidal cells in the frontal cortex send axons mainly to other ipsilateral/contralateral cortical areas.
Daniela Annibali et al.
PloS one, 7(7), e40269-e40269 (2012-08-01)
The transcription factor ID2 is an important repressor of neural differentiation strongly implicated in nervous system cancers. MicroRNAs (miRNAs) are increasingly involved in differentiation control and cancer development. Here we show that two miRNAs upregulated on differentiation of neuroblastoma cells--miR-9
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