N0513
Nerve Growth Factor-7S from murine submaxillary gland
lyophilized powder, suitable for cell culture
Synonyme(s) :
NGF-7S
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
A propos de cet article
Biological source:
mouse
Form:
lyophilized powder
Mol wt:
protein 130 kDa
Impurities:
≤10
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderService technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
Nerve Growth Factor-7S from murine submaxillary gland, NGF-7S, lyophilized powder, suitable for cell culture
biological source
mouse
Quality Level
form
lyophilized powder
potency
2-250 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
protein 130 kDa
packaging
pkg of 0.1 mg
technique(s)
cell culture | mammalian: suitable
impurities
≤10
color
white
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Ngf(18049)
General description
Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α2β2γ2 complex, where β2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.
Application
Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-
- PC12
- Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)
Biochem/physiol Actions
Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.
Physical form
Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.
Preparation Note
Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.
Analysis Note
The biological activity is measured using a 3-day MTT assay.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Kun-Shan Huang et al.
Evidence-based complementary and alternative medicine : eCAM, 2011, 109809-109809 (2009-08-19)
The present study provides in vitro and in vivo evaluation of Paeoniae alba Radix (PR) on peripheral nerve regeneration. In the in vitro study, we found the PR caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from
Hsien-Tung Chen et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 84(1), 256-262 (2007-05-22)
This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth
Héctor I Rocha-González et al.
Journal of neurophysiology, 100(1), 169-184 (2008-04-04)
Adult primary afferent neurons are depolarized by GABA throughout their entire surface, including their somata located in dorsal root ganglia (DRG). Primary afferent depolarization (PAD) mediated by GABA released from spinal interneurons determines presynaptic inhibition, a key mechanism in somatosensory
Sara Nencini et al.
Frontiers in physiology, 12, 644929-644929 (2021-08-03)
Piezo2 is a mechanically gated ion-channel that has a well-defined role in innocuous mechanical sensitivity, but recently has also been suggested to play a role in mechanically induced pain. Here we have explored a role for Piezo2 in mechanically evoked
Jinhuan Wei et al.
Journal of neuroinflammation, 19(1), 32-32 (2022-02-04)
Peripheral nerve injury (PNI) is a public health concern that results in sensory and motor disorders as well as neuropathic pain and secondary lesions. Currently, effective treatments for PNI are still limited. For example, while nerve growth factor (NGF) is
Contenu apparenté
Instructions
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique