N3520
Nutrient Mixture F-12 Ham
Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
Ham’s F-12
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Nutrient Mixture F-12 Ham, Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
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Application
Nutrient Mixture F-12 Ham is used for the following applications:
Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
- HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
- Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
- Used in media for the propagation of Cowdria ruminantium
- Used during preparation of primary mixed glial cultures
Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
General description
The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.
Preparation Note
Formulated to contain 11.1 grams of powder per liter of medium.
Supplement with 2.5 g/L sodium bicarbonate.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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