NA0400
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit
sufficient for 10 preparations
Synonyme(s) :
Gen Elute, GenElute Endotoxin-Free Plasmid Kit
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A propos de cet article
NACRES:
NA.52
UNSPSC Code:
41105501
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sufficient for 10 preparations
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
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technique(s)
DNA extraction: suitable
Quality Level
test parameters
sample volume: 150 mL bacterial culture
greener alternative category
storage temp.
15-25°C
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General description
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Biochem/physiol Actions
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.
Features and Benefits
- Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
- Only 35 minutes from pelleted cells to purified plasmid
- Convenient vacuum format
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- R4778Red Vac Cap 2
- W0263Wash Solution 1
- 2107E-Toxate™ Water, endotoxin, free
- W4639Wash Solution 2
- N7411Neutralization Solution EF
- C2112Column Preparation Solution
- R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Classe de stockage
10 - Combustible liquids
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