NXTRACT
NuCLEAR™ Extraction Kit
For mammalian tissue or cultured cells
Synonyme(s) :
Nuclear Isolation Kit
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A propos de cet article
NACRES:
NA.56
UNSPSC Code:
41105517
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Laissez-nous vous aiderusage
kit sufficient for 10 extractions (1 ml packed cell volume), kit sufficient for 100 extractions (100 μl packed cell volume)
technique(s)
protein extraction: suitable, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Catégories apparentées
General description
The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.
Application
NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.
Other Notes
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.
Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
Legal Information
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- 3× Dilution and Equilibration Buffer 90 mL
- P8340Protease Inhibitor Cocktail 1 mL
signalword
Danger
Classe de stockage
8A - Combustible corrosive hazardous materials
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
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Mounting evidence shows that nuclear factor-κB (NF-κB) plays an important role in endometriosis. We therefore evaluated the therapeutic potential of andrographolide, an NF-κB inhibitor. Primary cell cultures were performed using ectopic endometrial tissue specimens and their homologous eutopic endometrial specimens
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