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Merck

P2714

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder

Synonyme(s) :

Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
form:
lyophilized powder
solubility:
water: soluble
storage temp.:
−20°C

Nom du produit

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

biological source

bovine lung

form

lyophilized powder

solubility

water: soluble

storage temp.

−20°C

Quality Level

300

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Catégories apparentées

Application

Protease Inhibitor Cocktail powder has been used as a component:
  • in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
  • in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
  • in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
  • in 0.1 M NaCl solution to wash and collect skin secretions of frog

Biochem/physiol Actions

Inhibits serine, cysteine, and metallo-proteases.
The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Disclaimer

The lyophilized powder is stable for 4 years when stored at -20°C.

General description

The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.

Preparation Note

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000409166
0000409166
0000308021
0000308021
0000233200

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

  1. How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?

    One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

  2. How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?

    You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.  Aliquots can be stored at -20 C for 1 month.

  3. How much of Product P2714, Protease Inhibitor Cocktail, should I use with serum? 

    Dilute the contents of the vial in 10 mL of water, yielding a 10X initial concentration . Then dilute the stock 1:10 in the serum (1 part of inhibitor solution to 10 parts of serum). This would be a 1:100 dilution (containing the concentrations of each inhibitor as listed on the data sheet) in the serum. This is true for most cell lysates, serum, and culture media.

  4. Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?

    Yes, because it is water based.

  5. Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?

    No because it contains EDTA.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. What is the difference between Protease Inhibitors S8820 and P2714?

    S8820 is the tablet version of P2714.  

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Dennis SJ, et al.
Veterninary Research, 49(1), 1-6 (2018)
Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis
Wang M, et al.
Journal of biological and chemical chronicles, 155(1), 72-76 (2010)
F Maier et al.
Osteoarthritis and cartilage, 27(9), 1382-1391 (2019-05-24)
Given the structural changes associated with the progression of Osteoarthritis (OA), we hypothesized that patterns of through-thickness, large-strain shear evolve with early-stage OA. We therefore aimed to determine whether and how patterns of shear strains change during early-stage OA to
Giorgio Ramadori et al.
Nature communications, 10(1), 3545-3545 (2019-08-09)
Tens of millions suffer from insulin deficiency (ID); a defect leading to severe metabolic imbalance and death. The only means for management of ID is insulin therapy; yet, this approach is sub-optimal and causes life-threatening hypoglycemia. Hence, ID represents a
Céline Méhats et al.
PloS one, 3(10), e3445-e3445 (2008-10-23)
Prolonged neonatal exposure to hyperoxia is associated with high mortality, leukocyte influx in airspaces, and impaired alveolarization. Inhibitors of type 4 phosphodiesterases are potent anti-inflammatory drugs now proposed for lung disorders. The current study was undertaken to determine the effects
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