Accéder au contenu
Merck

P8999

Anti-p53 antibody, Mouse monoclonal

clone DO-7, purified from hybridoma cell culture

Synonyme(s) :

Anti-p53 antibody, Mouse monoclonal, Anti-TRP53, Anti-Transformation-related protein 53

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164709

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Anti-p53 antibody, Mouse monoclonal, clone DO-7, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DO-7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

isotype

IgG2b

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TP53(7157)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.
p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000506894
0000506894
0000332160
0000301489
0000301489

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Norm D Smith et al.
The Journal of urology, 169(4), 1219-1228 (2003-03-12)
An extensive body of literature regarding p53 has accumulated during the last 2 decades. The cellular mechanisms of p53 are complex yet well-defined, whereas its clinical usefulness in the management of bladder cancer remains controversial. We outline the basic constitutive
Ting Zhang et al.
International journal of molecular medicine, 40(1), 21-30 (2017-05-13)
Dihydroartemisinin (DHA) has been shown to inhibit the viability of various cancer cells. Previous studies have revealed that the mechanisms involved in the inhibitory effects of DHA are based on theactivation of p53 and the mitochondrial-related cell death pathway. However
Nalini Mehta et al.
Molecular therapy oncolytics, 4, 1-17 (2017-03-28)
Treatment of aggressive glioblastoma brain tumors is challenging, largely due to diffusion barriers preventing efficient drug dosing to tumors. To overcome these barriers, bacterial carriers that are actively motile and programmed to migrate and localize to tumor zones were designed.
Ruben Manuel Luciano Colunga Biancatelli et al.
Frontiers in physiology, 13, 812199-812199 (2022-04-08)
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused more than 5 million deaths worldwide. Multiple reports indicate that the endothelium is involved during SARS-Cov-2-related disease (COVID-19). Indeed, COVID-19 patients display increased thrombophilia with arterial and venous embolism and lung microcapillary
Predictors of progression in Barrett's esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression
Reid BJ, et al.
The American Journal of Gastroenterology, 96(10), 2839-2839 (2001)
Afficher tous les articles scientifiques apparentés

Contenu apparenté

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique