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Merck

P9582

Acide polyinosinique–polycytidylique potassium salt

with buffer salts, TLR ligand tested

Synonyme(s) :

Poly (I:C), Poly(I) • Poly(C)

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A propos de cet article

Numéro CAS:
31852-29-6
UNSPSC Code:
41106305
NACRES:
NA.51
MDL number:
MFCD00131983

Principaux documents

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Nom du produit

Acide polyinosinique–polycytidylique potassium salt, with buffer salts, TLR ligand tested

assay

≥99% (less than 1% free nucleotides, TLC)

form

lyophilized powder

composition

Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)

storage condition

desiccated

storage temp.

−20°C

Quality Level

300

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Application

TLR3 reconnaît l′ARN double brin et constitue un effecteur majeur de la réponse immunitaire contre les pathogènes viraux. L′acide polyinosinique–polycytidylique [Poly(I) • Poly(C)] est un homopolymère double brin utilisé comme ARN modèle pour étudier la signalisation cellulaire au niveau de TLR3. Poly(IC) est un ligand TRIF-dépendant du toll-like receptor-3 (TLR3).

Biochem/physiol Actions

La transfection de poly (I:C) dans les cellules NIT-1 a été utilisée comme modèle d′apoptose induite par les ARN double brin intracellulaires des cellules β. Dix-huit heures après la transfection, 45 % des cellules étaient apoptotiques, présentant une augmentation de la translocation de NF-kB, p50/p65 et un clivage des caspases 3 et 8, ainsi qu′une induction transcriptionnelle de la caspase 12, de Fas, d′IL-15 et de TRAIL (TNF receptor-associated ligand). Il a été suggéré que le poly(I:C) est l′un des meilleurs moyens d′obtenir des cellules dendritiques matures stables. Ces cellules dendritiques matures peuvent générer des réponses immunitaires efficaces in vitro après injection grâce à leur capacité à sécréter de l′IL-12 bioactive après la liaison de CD40. Le poly (I:C) a été utilisé comme adjuvant puissant pour stimuler les réponses immunitaires antitumorales spécifiques contre un vaccin à base de peptide.

Other Notes

Homopolymère double brin.

Packaging

Conditionnement en fonction de la teneur en polynucléotides.

Preparation Note

Ce produit nécessite une force ionique pour maintenir la structure en double brin. La reconstitution à environ 10 mg/ml d′eau produit un polynucléotide dans une solution physiologique tamponnée au phosphate.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000486506
0000486506
0000486507
0000486507
0000426474

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How stable is Product P9582, Polyinosinic-polycytidylic acid potassium salt, in solution?

    RNA and synthetic RNA analogues will be very stable in aqueous solution as long as the solutions are not contaminated with RNase.

  6. Should Polyinosinic-polycytidylic acid potassium salt Product P9582 or P1038 be used for cell activation?

    For cell activation, synthetic double-stranded RNA is widely used as ligand of Toll-like receptor 3 (TLR3) in place of what is supposed to be the natural ligand (viral dsRNA). The double stranded RNA homopolymer, i.e. Poly(I):Poly(C), Prod. No. P9582 can be used for this application.  P1038 is a single-stranded RNA and will not activate TLR3.

  7. What is the difference between Polyinosinic-polycytidylic acid potassium salt Products P9582, P1038, P1530 and P0913?     

    Prod No. P9582 as well as Prod. Nos. P1530 and P0913 are double-stranded with one strand of Poly(I) and one strand of Poly(C).  Prod. No. P1038 is a single-stranded RNA analog with C and I randomly making up the polynucelotide strand.  Double-stranded Prod. No. P9582 is a potassium salt while double-stranded Prod. Nos. P0913 and P1038 are sodium salts.  Prod. No. P0913 is gamma-irradiated.  None of the other products have been gamma-irradiated.  Unlike the other products, only approximately 10% of the  total mass of Prod. No. P9582 is actually polynucleotide.  The remaining 90% is buffer salts as sodium chloride and sodium phosphate.  

  8. What is the molecular weight of Product P9582, Polyinosinic-polycytidylic acid potassium salt?

    The molecular weight may vary from lot to lot with a range from 90,000 to 1,400,000 with an average of 200,000 to 500,000.     

  9. Why is the total mass of Product P9582, Polyinosinic-polycytidylic acid potassium salt, in my bottle much greater than the designated package size?

    The package size is based on the polynucleotide content.  However, since the polynucleotide is supplied with sodium chloride and sodium phosphate buffer salts, its mass represented by the package size is approximately 10% of the total mass present in the bottle.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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