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Merck

PP0100

Trypsin Profile IGD Kit

Synonyme(s) :

In gel digestion kit, Proteomics grade Trypsin, TPCK treated

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A propos de cet article

NACRES:
NA.56
UNSPSC Code:
12352200

Principaux documents

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usage

 kit sufficient for ≤100 applications

storage temp.

2-8°C

Quality Level

200

Catégories apparentées

General description

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Application

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.

Features and Benefits

  • Faster destaining than with alternative in-gel digest kits
  • Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
  • Compatible with Coomassie, SYPRO™ Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
  • Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.

Composants de kit seuls

Réf. du produit
Description
  • Destaining Solution Reconstituted to 75

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

3 - Flammable liquids

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup

wgk

WGK 2

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Certificats d'analyse (COA)

Lot/Batch Number
SLCQ8498
SLCJ8802
SLCD4484
SLCB1308
SLBW2626

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
Xingyuan Liu et al.
Cell death & disease, 13(7), 642-642 (2022-07-24)
Hepatocellular carcinoma (HCC) is the most common subtype of liver cancer and the second most fatal cancer in the world despite the great therapeutic advances in the past two decades, which reminds us of the gap in fully understanding the
Shuo Han et al.
Cell death & disease, 12(11), 1070-1070 (2021-11-12)
Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA
Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
SILAM for quantitative proteomics of liver Akt1/PKBa after burn injury.
Lu XM, et al.
International Journal of Molecular Medicine, 29, 461-461 (2012)
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