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Merck

R4642

Ribonucléase A from bovine pancreas

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Synonyme(s) :

RNAse a, RNase A, Ribonucléase pancréatique, Ribonucléate 3′-pyrimidino-oligonucléotido-hydrolase

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A propos de cet article

Numéro CAS:
9001-99-4
NACRES:
NA.53
UNSPSC Code:
12352204
MDL number:
MFCD00132181
Numéro CE :
3.1.27.5 (BRENDA, IUBMB)
Biological source:
bovine pancreas
Concentration:
20-40 mg/mL

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Nom du produit

Ribonucléase A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

SMILES string

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

biological source

bovine pancreas

grade

Molecular Biology

form

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

mol wt

13.7 kDa
 ~13,700

concentration

20-40 mg/mL

suitability

suitable for

foreign activity

Endonuclease and exonuclease, none detected
NICKase and DNase, none detected

storage temp.

−20°C

Quality Level

200

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Analysis Note

Protéine déterminée par E.

Application

  • La RNase A est utilisée pour supprimer l′ARN présent dans les préparations d′ADN plasmidique et d′ADN génomique ainsi que dans les échantillons protéiques.
  • Elle est également employée dans l′analyse de séquences d′ARN et les tests de protection.
  • Elle a également servi d′outil dans la conception de médicaments assistée par ordinateur.
  • La RNase A supporte l′analyse des séquences d′ARN.
  • Elle hydrolyse l′ARN contenu dans les échantillons protéiques.
  • La purification de l′ADN est supportée par la RNase A.
Suitable for:
  • RNase protection assays
  • Removal of unspecifically bound RNA
  • Analysis of RNA sequences
  • Hydrolysis of RNA contained in protein samples
  • Plasmid DNA purification

Features and Benefits

Notre ribonucléase A, ou RNase A, d′une grande stabilité, convient à l′élimination d′ARN, au séquençage d′ARN et à la purification d'ADN.

General description

La RNase A, ou ribonucléase A, est une endoribonucléase qui clive les liaisons phosphodiester de l′ARN simple brin après les nucléotides pyrimidiques. Elle attaque l′extrémité phosphate en 3′ (par exemple, la séquence pG-pG-pC-pA-pG est clivée en donnant pG-pG-pCp et A-pG). L′activité est maximale avec l′ARN simple brin. La RNase A est un polypeptide à chaîne unique contenant 4 ponts disulfure. Contrairement à la RNase B, ce n′est pas une glycoprotéine. Les ribonucléases n′hydrolysent pas l′ADN, car celui-ci est dépourvu des groupements 2′-OH essentiels à la formation d′intermédiaires cycliques. La RNase A peut également hydrolyser l′ARN présent dans les échantillons protéiques. Elle peut être inhibée par l′alkylation de His12 et His119 et est activée par les sels de potassium et de sodium. Cette RNase est inhibée en présence d′ions de métaux lourds. Elle est également inhibée par l′ADN par un effet de compétition.
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

Other Notes

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.

Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000485696
0000485695
0000485696
0000485697
0000485695

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution.  What does the solution contain?

    The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.

  3. What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)? 

    This product is stable for at least 2 years when stored properly at -20 °C. 

  4. When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?

    Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.

  5. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?

    The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Christopher P Selby et al.
The Journal of biological chemistry, 295(50), 17374-17380 (2020-10-23)
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Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6752-6757 (2017-06-14)
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R Perdigão-Henriques et al.
Oncogene, 35(2), 158-172 (2015-03-24)
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Recent large-scale studies revealed cell-type specific proteomes. However, protein complexes, the basic functional modules of a cell, have been so far mostly considered as static entities with well-defined structures. The co-expression of their members has not been systematically charted at
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Contenu apparenté

Data Sheet - R4642

Instructions

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