R8755
Milieu RPMI-1640
With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
Roswell Park Memorial Institute 1640 medium
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Milieu RPMI-1640, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Le milieu RPMI 1640 a été mis au point par Moore et ses collaborateurs, au Roswell Park Memorial Institute (RPMI), en 1966. Version modifiée du milieu 5A de McCoy, il a été formulé pour permettre la culture de cellules lymphoblastoïdes en suspension, mais on sait depuis qu'il convient aussi à une grande diversité de cellules adhérentes. Initialement conçu pour être utilisé avec un supplément à base de sérum, le milieu RPMI 1640 s'est révélé adapté à la culture sans sérum de différentes lignées cellulaires. Il est aussi couramment employé dans les protocoles de fusion ainsi que pour la croissance de cellules hybrides. Ce milieu convient à la culture de leucocytes normaux et néoplasiques humains.
Application
RPMI-1640 Medium is used for the following applications:
- Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
- Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
- Used for cell isolation and culture
- Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
- Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
- Used for the preparation of antifungal agents
Preparation Note
Formulated to contain 10.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.
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Classe de stockage
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
R Roubenoff et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 53(1), M20-M26 (1998-02-19)
To determine the association among aging, inflammation, and cytokine production by peripheral blood mononuclear cells. We examined production of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), IL-1 receptor antagonist (IL-1Ra), and IL-6 in 711 elderly participants in the Framingham
M E Klepser et al.
Antimicrobial agents and chemotherapy, 41(6), 1392-1395 (1997-06-01)
Time-kill curves were determined for three isolates of Candida albicans tested against fluconazole and amphotericin B at multiples of the MIC. Fluconazole produced fungistatic activity, with concentration-related growth effects observed over a narrow range of concentrations. Amphotericin B exhibited fungicidal
M Farrington et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1099-1103 (1994-02-01)
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play